Essential Reading! Get my 3rd book: Eat Their Lunch “The first ever playbook for B2B salespeople on how to win clients and customers who are already being serviced by your competition.” Buy Now Over time, no matter how good you are, no matter how much you care, you will lose clients.You will lose some clients through no fault of your own.You will be doing everything right, and they will change their strategy, eliminating the need for what you provide them.You will be producing the necessary outcomes, and your client will be purchased by another company, and that company will already have a strategic partner in your spot.Some of the people with whom you do business will leave to pursue new opportunities. Many of them will take you with them. But their replacements may have their own relationships, and you too will be replaced.You will lose some clients and it will be your fault (If it’s your company’s fault, it is still your fault. You own it.)You will do everything in your power to succeed in delivering the outcomes you sold, and for whatever reason, it just won’t work out.You will face challenges that you didn’t expect, and try as you may, you won’t be able to overcome those challenges.Sometimes you will greatly underestimate how much resistance there is to change, and you will not have the support you need inside your client’s company to effect the change they need.Not to worry, none of this will be a frequent occurrence. But neither will it be at a time of your choosing. There is only one thing that you can do to be prepared for the inevitable client loss that comes with being a sales organization: build your pipeline.The only thing you can rely on to ensure you go ever upwards and never backwards is to insure yourself against losses. The price you pay for that insurance is a strong pipeline of dream client opportunities and the ability to create value for them. When the inevitable loss occurs, it will be too late to start building.QuestionsWhy did you lose the last client you lost?Why did you lose the last account you lost when it wasn’t your fault or responsibility?How do you insure against these losses?Is your pipeline offering you the protection you need now?
You can’t manage your time. Time is something over which you have zero control. You can’t find more time because there isn’t any more. You have all you are going to have, do with it what you may.Even though you can’t find or make more time, you can exercise control over yourself. This isn’t time management; it’s “me management.” If you want to get the most out of the time you have, you have to become an excellent “me manager.”Me management is about ruthless prioritization.You can’t do all you would like to do in a single day. Add a week’s worth of single days together and you have even more you wish you had time to do. The only way to be as effective as you need to be is by prioritizing. Ruthlessly.When you are doing something, you are not doing everything else you want–or need–to do.Not What. Who?I am completely and totally task driven. But it isn’t what you are doing that matters most. It’s for whom you are doing it. If you are going to ruthlessly prioritize your time, you need to start with the outcomes you need to generate for and with the people who matter most in your life (and your business life is a big part of your life, isn’t it?).People are always first, but even they need to be prioritized.Who matters most? Time you spend with some is time you aren’t spending with others. Think it through.Now What?Once you’ve taken care of prioritizing the outcomes you need to produce for the most important people in your life, you can look downstream at the “what.” What are the three or four biggest and most important outcomes you need to achieve in a day? How do those outcomes move you closer to your vision, your mission, your goals?If what you are doing doesn’t move you closer to your goals, you need to ruthlessly find a way to avoid that work. Delegate it. Defer it. Delete it. Automate it.Remember, you aren’t going to have any more time. This is it, baby, make it count!Just Say No.Let’s get ruthless . . . in a good way. You have to say “no” to a lot of things you want to say “yes” to. You have no choice in this matter. If you say “yes” to something, you are necessarily saying “no” to something else.Ask yourself, “By saying ‘yes’ to this request, what am I going to be saying ‘no’ to?” Are you willing to say “no” to something bigger because you are afraid to say “no” to something smaller? Are you comfortable occasionally disappointing others because you have to serve the people and the outcomes that make up your bigger purpose, your meaning?You pay for success in advance. Ruthless prioritization is part of that price. Essential Reading! Get my 3rd book: Eat Their Lunch “The first ever playbook for B2B salespeople on how to win clients and customers who are already being serviced by your competition.” Buy Now
Podcast: Play in new window | Download (Duration: 21:52 — 20.0MB)Subscribe: Apple Podcasts | Android | Email | Google Podcasts | RSSToday’s conversation has Anthony really scared simply because it’s with a startup founder who has a technology product – not exactly Anthony’s strong suit. But he’s daring to jump into the conversation and actually does a great job chatting with Steli Efti, founder of Close.io – a software solution that enables sales people to work with their prospects, leads, and customers in a way that is intuitive for the salesperson and easy to use. You’ll hear Steli tell a bit about his own journey and the how he’s navigated the entrepreneurial path to where he’s at now. It’s a great conversation, even though Anthony was a bit scared at first.Just because something CAN be done doesn’t mean it will be a successful product ~ Steli EftiClick To TweetWhen a startup gets going they typically make some fundamental mistakes when it comes to sales.Founding and running a successful startup is hard, no question. But many times the networking and reliance upon connections cause startup founders and leaders to miss some of the more important parts of how sales work and how the sales process should be integrated into every aspect of the brand and its promotions. On this episode Steli Efti shares the problems of that nature that he saw in the startup community of Silicon Valley and how it led him to create a software solution for sales teams that is entirely different than what’s out there.Why you NEED to make mistakes as a startup founder or entrepreneur.Steli Efti’s path to being the founder of a successful startup was not a bed of roses. He’s had his share of failed ventures and lessons learned from mistakes. He believes that every entrepreneur not only will make mistakes but needs to make mistakes so that they can learn things on a deeper level that will serve them later on. Anthony and Steli chat about the importance of learning from mistakes and why you can’t allow your mistakes to define you, on this episode of In The Arena.If you are in sales, you have to be ready for failure and learn from it ~ Steli EftiClick To TweetJust because it CAN be done, doesn’t mean it will be a successful productMuch of what passes for email marketing is nothing more than SPAM – lampe attempts at prospecting through form emails and repeated systems. Steli Efti says that many entrepreneurs are taking that approach simply because it’s what has always been done – but it neglects to notice that methods of that kind aren’t working anymore. Steli and Anthony both share insights into what works better and how and why marketers and salespeople need to sit across the table from real people in order to do sales the right way. It’s a transition that could change the way you do sales, on this episode.Sales software that works intuitively for the salesperson.For far too long sales, prospecting, and CRM software has been too cumbersome and clunky, causing the salesperson to have to call the software engineer to get the software to do what they need it to do. But Steli Efti’s company, Close.io has changed all that with it’s new software solution. Its ease of use is based on the salesperson’s needs and allows all kinds of variations and filters to be integrated into the workflow so that the data that is needed is easily found. It also integrates directly into communication solutions so there’s less time wasted and more time spent dealing directly with prospects. Hear about the software and learn what it can do for you, mon this episode.Sales software that thinks like a #salesperson, on this episode of In The Arena Click To TweetOutline of this great episode Why Anthony is scared about bringing Steli onto the show today. Who is Steli Efti? The challenges Steli saw regarding sales and startups. The first thing Steli worked on that didn’t work out. Mistakes startups were making regarding sales. Why it’s not a product unless you can create a customer. The problem Steli was trying to solve with his software solution. Eliminating data entry and increasing communication for salespeople. How you canResources & Links mentioned in this episodewww.SalesForce.comwww.Close.io – Steli’s company websiteSteli’s on TwitterThe theme song “Into the Arena” is written and produced by Chris Sernel. You can find it on SoundcloudConnect with AnthonyWebsite: www.TheSalesBlog.comYoutube: www.Youtube.com/IannarinoFacebook: https://www.facebook.com/iannarinoTwitter: https://twitter.com/iannarinoGoogle Plus: https://plus.google.com/+SAnthonyIannarinoLinkedIn: https://www.linkedin.com/in/iannarinoTweets you can use to share this episodeYou can’t allow failure to impact your identity if you’re an entrepreneur ~ Steli EftiClick To TweetIt’s not a product unless you can create a customer ~ Anthony IannarinoClick To TweetSubscribe toIn the ArenaApple PodcastsGoogle PodcastsAndroidby EmailRSSOr subscribe with your favorite app by using the address below
Essential Reading! Get my 2nd book: The Lost Art of Closing “In The Lost Art of Closing, Anthony proves that the final commitment can actually be one of the easiest parts of the sales process—if you’ve set it up properly with other commitments that have to happen long before the close. The key is to lead customers through a series of necessary steps designed to prevent a purchase stall.” Buy Now The reason so many salespeople dislike their CRM and the necessary upkeep is that they believe that it is Big Brother. There is some truth in this complaint, and for some sales leaders and managers, sales can boiled down to activity alone—effectiveness and effective leadership be damned. Other “desk jockey” types sit behind the sophisticated dashboard, clicking through to reports and immersing themselves in the details like a military commander in the Rear Echelon, so far from the action that they are unaware of the reality, the ground truth.For all the reports and reporting, none of them by themselves or combined, generate the real outcome all sales organizations are seeking: accountability. Information generates only an awareness, and is insufficient to the task of creating greater accountability and the reaching of goals (and I say this after spending an entire day generating reports).AccountabilityAccountability, for many reasons, is a problem for most sales organizations. Some of the lack of accountability is due to legal constraints making it more difficult to impose consequences—even for bad or detrimental behavior. Another reason is that the accountability doesn’t cascade down from senior leadership to the level beneath them, often the result of modeling their prior leaders, and occasionally the result of coming from a culture that was high accountability and assuming everyone has the same culture (something rarer than an honest politician).But there is a more difficult and more prevalent reason for the lack of accountability: the inability or unwillingness to create change.No Fear of ReportsSalespeople aren’t afraid of reports. They also aren’t afraid of dashboards. As Steven Pressfield wrote in The Warrior Ethos: “Sociologist tell us that there are two types of cultures: guilt-based and shame-based. Individuals in a guilt-based culture internalize their society’s conception of right and wrong. The sinner feels his crimes in his guts. He doesn’t need anyone to convict and sentenced him; he convicts and sentences himself.”In a shame-based culture, “face” is everything. Pressfield continues: “A shame based culture imposes its values from outside the individual, by the good or bad opinion of the group. The community imposes its code on its members by shunning and public shaming.” Shame works in military groups where it is critical to success, but Pressfield will tell you that here in the West, we are guilt-based, making your shared dashboard impotent in creating change.A culture that lacks accountability does not create an internal sense of obligation and purpose and meaning. I would argue that there is no real feeling of “belonging” and “duty” and “obligation.” Nor is leadership the exemplar.
With three days to go for the famous Rath Yatra of Lord Jagannath, final touches are being given to the preparations for the grand event at the coastal town of Puri on June 25. Every year, the Jagannath Rath Yatra is witnessed by lakhs of devotees from all corners of the world.Three chariots have already been constructed for the deities — Lord Balabhadra, Lord Jagannath and Devi Subhadra. Traditional artists are painting fresh and glowing colours on different idols.The body of the three chariots which will be draped with multicoloured cloth two days before the Rath Yatra.On the eve of the Rath Yatra, devotees can catch a glimpse of the three deities dressed gorgeously on the occasion of the Naba Jaubana Besha Darshan. In accordance with traditional rituals, the deities can be seen for the first time after the gap of a fortnight over which they remain secluded in the anasara ghaa (retiring room) of the 12th Century temple. The Shree Jagannath Temple Administration (SJTA) is expecting a huge turnout of devotees for the day.“There will be CCTV surveillance on rituals that will be performed outside the temple. Nobody, including the servitors, would be allowed to touch the idols. The administration is in constant touch with the servitors to ensure the Rath Yatra passes off peacefully,” said Laxmidhar Pujapanda, a spokesperson for SJTA.Meanwhile, State police is gearing up to have a massive security cover in place for the event.
A woman was killed and a soldier injured in two incidents of ceasefire violation and militant attack on Saturday in Jammu and Kashmir.An official said Rakia (45) was killed after she was hit by a mortar shell fired from Pakistan in Mendhar area of Poonch on Saturday morning.Indian Army personnel guarding the border posts retaliated strongly and effectively.Around 0520 hours, mortar shells fired from across the border exploded near the house of Mohd. Shabir at Gohlad Kalran village, killing Rakia, a police officer said. The exchange of fire stopped at 0645 hours, he said.On August 8, the Pakistani troops resorted to firing and shelling in the Krishnagati sector of Poonch district, killing Sepoy Pawan Singh Sugra (21).Till August 1 this year, there have been 285 instances of ceasefire violation by the Pakistani forces. In 2016, the number was significantly less at 228.Militants fire at Army camp in J&K, jawan injuredIn a separate incident, a soldier was injured after militants attacked an Army camp in forest area of Kalaroose in frontier Kupwara on Friday late night.A group of militants attacked the Army’s 41 RR headquarter camp, which is located in upper belt of Kalaroose forests along the Line of Control.After 10 minutes of firing, a police official said, the militants fled from the area.One soldier of 21 J&K Rifles was injured and he was shifted to the military hospital at Drugmulla.
After two days of protests and clashes with the police, the situation in Udaipur city in south Rajasthan remained peaceful on Saturday, though prohibitory orders were in force and mobile internet services continued to remain suspended. More than 40 persons, including two dozen policemen, were injured in the violence.Despite the prohibitory orders, right-wing groups including the Vishwa Hindu Parishad and Bajrang Dal organised rallies on the city’s roads for two days in support of Shambhu Lal Raigar, who had hacked to death a Muslim migrant labourer from West Bengal in Rajsamand last week. The organisers claimed that their protest was against “inflammatory slogans” raised earlier by Muslims.During the protests, an incident of a man climbing on the roof of the district court building and unfurling a saffron flag created a flutter in the town. As the youth waved the flag, the protesters argued and clashed with the policemen while trying to prevent them from entering the court premises.Of the 220 persons detained by the police, 53 were formally arrested on charges of rioting, unlawful assembly, causing damage to public property and obstructing public servants from carrying out duty and assaulting them. While the 53 accused were remanded in judicial custody on Saturday, others were released on personal bond.Inspector-General of Police Anand Srivastava said prohibitory orders would continue for a couple of days, depending on the situation. The man who unfurled the saffron flag on the court building was yet to be identified.Crowds had gathered throughout the day on Thursday in different areas of the city, including Town Hall, Chetak Circle, Madhuban and Fatehpura, but the police dispersed them by use of force. The mob went out of control when it reached Court Circle and got support from the lawyers, after which the clashes with policemen took place.Police have also arrested 10 persons who participated in a demonstration organised by the Muslim Mahasabha at Chetak Circle in the town on December 8 and allegedly raised some provocative slogans. They were demanding stringent punishment to Raigar for killing the labourer, Mohammed Afrazul.
Come Gudi Padwa, the Marathi New Year on March 18, Maharashtra will get tough on plastic ban violators. Environment Minister Ramdas Kadam on Friday announced a ban on specified plastics in the State.“Plastic bags, thermocol, disposable cups and plates, cutlery, non-woven polypropylene bags, plastic pouches and packaging would be banned,” said Mr. Kadam. Violators would be fined ₹25,000 and could also face a three-year jail term. The ban covers the production, use, storage, sale, distribution, import and transport of plastic, Mr. Kadam told the State Assembly.Plastic milk pouches must be above 50 microns, and be recyclable. Pouches should state the cost of recycling and customers can claim a refund of 50 paise per pouch. PET bottles would be recycled at not more than ₹1 per bottle under a new plan.
Environmental NGO Goa Foundation filed a petition in the Bombay High Court at Goa earlier this week, seeking coordination among government agencies on the issue of safety in mines during the monsoon.The foundation has requested the court to set up a three-member committee comprising representatives of the Indian Bureau of Mines, Director of Mines Safety, and Goa Directorate of Mines and Geology. Monsoon riskSaying that there is a danger of mine collapses during monsoon, the NGO said the government agencies would take urgent action in the matter only if tasked by the High Court. The mines are currently closed as per a Supreme Court order.Claude Alvares, director of Goa Foundation, said in the petition that an affidavit filed by the State government earlier said it was in no position to carry out safety work in all the mining areas of the State.The foundation said, “Some of the mine pits are on hillsides, and if they are not looked after and accumulated water is not discharged, there are chances of mine collapse.” It said such collapses have affected people and property, including agricultural fields, in valleys in the past. The petition said the Director of Mines Safety has told court that all activities related to danger during the monsoon “should be monitored continuously by well qualified and competent persons in field of mining.” However, he does not volunteer any assistance, the foundation said.
Misinterpretation of legal provisions has cost the Haryana government ₹2.87 crore in the last eight years, and the authorities are now in the process of recovering the amount from former and current legislators who have unknowingly benefited from the “error”.The State government paid ₹2.87 crore towards the income tax of present and former legislators between 2010-11 and 2017-2018, without realising that the amount was to be borne by the MLAs, as per the Haryana Legislative Assembly (Salary, Allowances and Pensions of Member) Act, 1975.The Vidhan Sabha Secretariat has now initiated the process of recovering the amount from around 140 MLAs after calculating the exact amount each legislator is required to pay.“We have calculated the tax of each MLA, including the former legislators. The recovery process has been started and we are deducting ₹20,000 per month. The deduction commenced from the salary of May, which was paid in June,” said an official privy to the matter.As for those MLAs who are drawing their salary from January 1, 2018, income tax to the tune ₹7,000 monthly is being deducted now, the official added. RTI queryThe “goof-up” came to light when Hemant Kumar, a lawyer at the Punjab and Haryana High Court, filed an application under Right to Information Act with State Public Information Officer of Haryana Vidhan Sabha Secretariat, seeking information on the income tax paid by the State government towards its MLAs.Mr Kumar had sought to know whether the State was paying income tax on both the “salary and the allowances” of the MLAs, and if that was so, then what was the total amount paid since 2010-11 till date.In its reply, the SPIO, in March this year, admitted that income tax on the salary as well as allowances of MLAs had been paid “inadvertently by misconstruing Section 8 of Haryana Legislative Assembly (Salary, Allowances and Pensions of Member) Act, 1975”.“The Act clearly states that only the MLAs’ allowances and not their salary shall be exclusive of the tax payable in respect thereof under any law relating to income tax for the time being in force and such tax shall be borne by the State government,” said Mr. Kumar.He said that while in its first reply the government said ₹48.14 lakh was mistakenly paid towards the income tax on salaries of MLAs, later, it revised the amount to ₹2.87 crore.
A man was beaten to death allegedly by three brothers on suspicion of cattle theft at Bahrola in Palwal district. The deceased is yet to be identified.The victim was caught by Ramkishan and his brothers Prakash and Bir Singh in the early hours of Friday when he, along with his two others, allegedly tried to steal their buffaloes. He was badly beaten up and later died. A case has been registered at the Sadar police station. Ramkishan has been arrested.“Efforts are being made to arrest Prakash and Bir Singh,” said Palwal Superintendent of Police Waseem Akram.
The Kamrup Metropolitan District Magistrate on Wednesday ordered an inquiry into the death of a person, who had allegedly escaped from the police custody and was found hanging from a tree in Chandrapur area. District Magistrate Virender Mittal directed Chandrapur Revenue Circle Officer Pallab Jyoti Nath to conduct an inquiry to find out the circumstances leading to the death of the 23-year-old man, official sources said here.Mr. Nath has been directed to submit his findings within 15 days for onward submission to the NHRC/AHRC (Assam Human Rights Commission).‘Harassed girl’ The person, identified as Chandan Bharali, was arrested on Tuesday for allegedly harassing a girl on social media and was taken to Panikhaiti outpost after being remanded in police custody for three days by the Chief Judicial Magistrate.The accused was found missing on Tuesday night and the police claimed that he had escaped with his handcuffs. A search was launched and the police found him early on Wednesday morning hanging from a tree in the jungle area, about 150 metres behind the Panikhaity outpost. Bharali had allegedly committed suicide, according to the police.
The Maharashtrawadi Gomantak Party (MGP), a BJP ally in Goa, gave an ultimatum on Saturday that the charge of the Chief Minister’s post be given to its minister Sudin Dhavalikar in view of Manohar Parrikar’s illness. The MGP warned that it would contest the Lok Sabha polls and Assembly by-elections in the state against the BJP candidates if the demand was not met.Mr. Parrikar, who is suffering from a pancreatic ailment, is recuperating at his residence since October 14 after being discharged from the All India Institute of Medical Sciences (AIIMS), New Delhi. Two Assembly seats — Mandrem and Shiroda — fell vacant last month after respective MLAs, Dayanand Sopte and Subhash Shirodkar, resigned as legislators and quit the Congress to join the BJP. “Party’s central committee had a detailed discussion on the leadership issue today. Chief Minister Manohar Parrikar is ailing and, because of that, the administration is completely paralysed for the last eight months,” MGP president Dipak Dhavalikar said. “Therefore, to bring in discipline and efficiency to the administration, we demand that the chief minister’s charge be given to senior MGP MLA Sudin Dhavalikar as early as possible,” he said. “If the charge is not given immediately, we will contest all upcoming elections, that is (Assembly) by-election and Lok Sabha polls, by fielding our candidates against official candidate of the government,” he said. Sundin Dhavalikar is presently the second-most senior minister in Goa Cabinet after Mr. Parrikar. MGP’s central committee will meet again next month to review the situation, Mr. Dipak Dhavalikar said. “We will see if the government has taken our warning seriously or has continued with the same situation,” he said, adding that he himself might contest the by-election from Shiroda, while the party would support Independent MLA Jit Arolkar in Mandrem. MGP is part of the BJP-led coalition, which also includes Goa Forward Party and three Independents.
Ahead of Prime Minister Narendra Modi’s scheduled visit to Baripada on January 5, Odisha Chief Minister Naveen Patnaik on Thursday countered his allegations about the BJD government’s failure in achieving promised irrigation targets in the State. Addressing a public meeting at Khurda near Bhubaneswar on December 24, Mr. Modi had said that the BJD government had promised to extend irrigation to additional 10 lakh hectares of land, but only 22,000 hectares had been irrigated. “There was a huge gap between promise and delivery,” Mr. Modi had alleged.“I am happy to share that since 2014, Odisha has created an additional irrigation potential of 7.8 lakh hectares and we are on course to surpass the 10 lakh hectares target that my government has set for itself,” Mr. Patnaik said in a letter to Mr. Modi.Irrigation potentialStating that Odisha is among the top States in the country in investment in irrigation and creation of irrigation potential, Mr. Patnaik said: “From 2014, an amount of ₹26,889 crore has been spent in irrigation and water sector with contribution of ₹1,211 crore that is 4.5% from the government of India contribution through PMKSY (AIBP). In fact, in the current year, a record amount of ₹10,196 crore has been provided by the State.” The Chief Minister further pointed out that with its own programme 46 mega lift irrigation projects with ayacut 54,472 hectares, 53,576 deep bore wells with ayacut 2,67,880 hectares, 5837 community lift points with ayacut 1,29,764 hectares and 8335 check dams with ayacut 97,694 hectares have been created.“The detailed information on creation of irrigation potential from April 2014 to November 2018 is also available in our website www.dowrodisha.gov.in,” Mr. Patnaik said.The letter of Mr. Patnaik contradicting the Prime Minister’s statement assumes significance ahead of the Prime Minister’s visit to Baripada to inaugurate several projects and address a public meeting. According to political analysts, Mr. Patnaik’s letter to Mr. Modi was an attempt to checkmate senior BJP leaders from creating any confusion among the people about the BJD government’s performance in Odisha. This is the first ever direct and formal counter by Mr. Patnaik to the BJP ahead of the forthcoming elections to the Lok Sabha and the Assembly in the State.
Folk song competitions, quiz programmes and appeals by celebrities are some of the initiatives being undertaken by the election office in Assam for attracting women, first-time voters, people with physical disabilities and senior citizens to cast their votes.Sprinter Hima Das, Whitley Awardee and environmentalist Purnima Devi Barman, singer Tarali Sarma, body builder Golap Rabha and actor Kopil Bora have been roped in as State icons to create awareness about the election process. District election officers have been directed to reach out to the maximum number of voters in the constituencies under their jurisdiction, Assam Chief Electoral Officer Mukesh Sahu said. “We have kept the local aspects in mind while chalking out the programmes under the Systematic Voters’ Education and Electoral Practices and our aim is to reach out to all sections of voters,” Mr. Sahu said.‘Make informed choice’Ms. Das, herself a first-time voter, has urged young voters in a promotional video, to actively participate in the election. Tarali Sarma, who sang the theme song in another awareness video, is seen appealing to first-time voters to make “informed and ethical choices” while casting their “precious” vote. Besides organising cycle rallies and street plays, campus ambassadors are being appointed in colleges for holding chunaav pathshalas to spread the awareness among first-time voters through co-curricular activities. In Kamrup district, an election quiz was organised for students while 200 students of IIT-Guwahati have volunteered to undertake door-to-door campaigns in the interior areas, a district election official said. A major component of the SVEEP is to remove gender gap in polls and an initiative ‘Aaideur Chora’ has been taken up in collaboration with the Assam State Rural Livelihood Mission and Assam State Urban Livelihood Mission to sensitise female voters, the CEO said. The ASRLM and ASULM are mobilising self-help groups to open women help desks at various government outlets like gram panchayat offices and cooperative societies to facilitate women voters to verify their names, go through voters’ lists and make corrections, if needed. They can also register their names if these do not appear on the rolls. ‘Chandraprabha’, a woman mascot, will be used for spreading the message of participation in the local language. The nodal officers of the SVEEP campaigns are organising ‘naam’ (folk song) singing, ‘pitha’ and ‘laru’ (Assamese sweets) making competitions and street plays to involve women in the awareness campaigns. Polling stations with low female voter turn-out have been identified and ‘mahila voter rallies’ are being held, the CEO said. Mr. Sahu said that persons with disabilities are a major area of focus.
Entamoeba histolytica is a tiny pathogen that takes a terrible toll. The single-celled parasite—an amoeba about a tenth the size of a dust mite—infects 50 million people worldwide and kills as many as 100,000 each year. Now, a new report reveals how the microbe does its deadly damage: by eating cells alive, piece by piece. The finding offers a potential target for new drugs to treat E. histolytica infections, and it transforms researchers’ understanding of how the parasite works.“This process of nibbling of cells went unrecognized by everyone in this field, including me, for over a hundred years,” says infectious disease specialist William Petri of the University of Virginia in Charlottesville, a co-author of the report who has studied E. histolytica for more than 2 decades.Although scientists have studied E. histolytica for more than a century, much about the parasite remains a mystery. Part of the problem is that it behaves unpredictably. Many of those infected show no symptoms at all—the amoeba lives quietly in their gut, feeding on bacteria without causing trouble. But in others, the parasite attacks the gut itself and can cause potentially fatal diarrhea, intestinal ulcers, and liver abscesses. This illness, called amebiasis, is a leading cause of parasitic death among humans. Common in parts of the developing world, including Africa, Latin America, and South Asia, it is transmitted via contaminated food and water. But researchers knew only bits and pieces of how the disease plays out in the gut. They knew, for example, that the amoeba killed only cells with which it had direct contact, and that it bound itself to those cells using specific sugars, called lectins.Sign up for our daily newsletterGet more great content like this delivered right to you!Country *AfghanistanAland IslandsAlbaniaAlgeriaAndorraAngolaAnguillaAntarcticaAntigua and BarbudaArgentinaArmeniaArubaAustraliaAustriaAzerbaijanBahamasBahrainBangladeshBarbadosBelarusBelgiumBelizeBeninBermudaBhutanBolivia, Plurinational State ofBonaire, Sint Eustatius and SabaBosnia and HerzegovinaBotswanaBouvet IslandBrazilBritish Indian Ocean TerritoryBrunei DarussalamBulgariaBurkina FasoBurundiCambodiaCameroonCanadaCape VerdeCayman IslandsCentral African RepublicChadChileChinaChristmas IslandCocos (Keeling) IslandsColombiaComorosCongoCongo, The Democratic Republic of theCook IslandsCosta RicaCote D’IvoireCroatiaCubaCuraçaoCyprusCzech RepublicDenmarkDjiboutiDominicaDominican RepublicEcuadorEgyptEl SalvadorEquatorial GuineaEritreaEstoniaEthiopiaFalkland Islands (Malvinas)Faroe IslandsFijiFinlandFranceFrench GuianaFrench PolynesiaFrench Southern TerritoriesGabonGambiaGeorgiaGermanyGhanaGibraltarGreeceGreenlandGrenadaGuadeloupeGuatemalaGuernseyGuineaGuinea-BissauGuyanaHaitiHeard Island and Mcdonald IslandsHoly See (Vatican City State)HondurasHong KongHungaryIcelandIndiaIndonesiaIran, Islamic Republic ofIraqIrelandIsle of ManIsraelItalyJamaicaJapanJerseyJordanKazakhstanKenyaKiribatiKorea, Democratic People’s Republic ofKorea, Republic ofKuwaitKyrgyzstanLao People’s Democratic RepublicLatviaLebanonLesothoLiberiaLibyan Arab JamahiriyaLiechtensteinLithuaniaLuxembourgMacaoMacedonia, The Former Yugoslav Republic ofMadagascarMalawiMalaysiaMaldivesMaliMaltaMartiniqueMauritaniaMauritiusMayotteMexicoMoldova, Republic ofMonacoMongoliaMontenegroMontserratMoroccoMozambiqueMyanmarNamibiaNauruNepalNetherlandsNew CaledoniaNew ZealandNicaraguaNigerNigeriaNiueNorfolk IslandNorwayOmanPakistanPalestinianPanamaPapua New GuineaParaguayPeruPhilippinesPitcairnPolandPortugalQatarReunionRomaniaRussian FederationRWANDASaint Barthélemy Saint Helena, Ascension and Tristan da CunhaSaint Kitts and NevisSaint LuciaSaint Martin (French part)Saint Pierre and MiquelonSaint Vincent and the GrenadinesSamoaSan MarinoSao Tome and PrincipeSaudi ArabiaSenegalSerbiaSeychellesSierra LeoneSingaporeSint Maarten (Dutch part)SlovakiaSloveniaSolomon IslandsSomaliaSouth AfricaSouth Georgia and the South Sandwich IslandsSouth SudanSpainSri LankaSudanSurinameSvalbard and Jan MayenSwazilandSwedenSwitzerlandSyrian Arab RepublicTaiwanTajikistanTanzania, United Republic ofThailandTimor-LesteTogoTokelauTongaTrinidad and TobagoTunisiaTurkeyTurkmenistanTurks and Caicos IslandsTuvaluUgandaUkraineUnited Arab EmiratesUnited KingdomUnited StatesUruguayUzbekistanVanuatuVenezuela, Bolivarian Republic ofVietnamVirgin Islands, BritishWallis and FutunaWestern SaharaYemenZambiaZimbabweI also wish to receive emails from AAAS/Science and Science advertisers, including information on products, services and special offers which may include but are not limited to news, careers information & upcoming events.Required fields are included by an asterisk(*)“We thought they somehow caused the cells to die, and then the parasites would eat the dead cells,” says microbiologist Katherine Ralston of the University of Virginia. Because amoebae are known to feed by phagocytosis—a process in which one cell engulfs and “eats” another—researchers assumed the amoebae ingested the dead cells whole.But Ralston wondered if new live microscopy techniques, which enable scientists to capture video of cells in action, might reveal more. Working with Petri and colleagues from the University of Virginia and Jawaharlal Nehru University in New Delhi, Ralston put the parasitic amoebae and human cells that were made fluorescent so they would be easier to spot in a dish and examined their interaction. What the team saw surprised them.“It was remarkable to see the amoebae were taking bites,” Ralston says. Within one minute of contact, the parasites were ripping off and ingesting fragments of the human cells, which were visible as bits of fluorescent material within the amoebae. The biting resembled trogocytosis, a process in which immune cells extract bits of other immune cells, but was unique in that it occurred between a parasite and its host and ultimately caused cell death. Once an amoeba took its first bite, it continued to consume more and more of the cell until the cell died about 10 minutes later.“A little bit of nibbling caused more nibbling,” Ralston says, “and this was happening while the human cells were alive.”When the cells were dead, the amoebae stopped nibbling, detached from the cells, and moved on. Human red blood cells faced the same gruesome end. (See video—the human cell appears in pink, the amoeba in green.) The team then repeated the experiment with live intestinal tissue from mice engineered to have fluorescent intestinal cells and found the parasites invaded this tissue and caused damage similar to that seen in samples of E. histolytica-infected human colon tissue.The researchers couldn’t be sure that the nibbling was actually causing the cell deaths, however, so they used a drug that interferes with the amoeba’s ability to reshape itself for biting to observe its impact on cell death. The drug-hampered amoebae nibbled less and did not kill the cells. Neither did amoebae the team genetically modified, so they could not produce the proteins and lectins often found where the parasites make contact with human cells.Together, the results suggest that this piecemeal nibbling drives the cell death and damage sometimes seen in E. histolytica’s wake, and that the lectins may induce or regulate the biting behavior, the team reports online today in Nature. If additional studies confirm that E. histolytica actually kills cells this way in people suffering from amebiasis, Ralston says, they could point the way to new treatments for the illness. “If we could understand how the amoeba takes a bite, that would be a good target for therapeutic drugs.”The team’s discovery could change the game for E. histolytica research, says Upi Singh, a physician and infectious disease researcher at Stanford University in California who was not involved in the study. “It’s really fantastic for the field to have a study of this caliber come along,” she says. “This changes the paradigm.”
Artificial sugars aren’t so sweet for fruit flies. The insects live an average of 45 to 60 days, but those raised in tubes containing Truvia (pictured)—one of the best-selling sugar substitutes in the United States—lived for an average of only 5.8 days. Six other sweeteners—four artificial, two natural—had no impact on lifespan, the team reports online today in PLOS ONE. Researchers also found that Truvia-fed flies had difficulty in climbing up a small vial, indicating impaired motor function. The problem, the team discovered, lies in an ingredient present in Truvia but not in the other six sweeteners: erythritol, a commonly used food additive approved by the U.S. Food and Drug Administration. This is the first evidence that erythritol baits could be used as effective insecticides, at least against fruit flies, say the researchers.*Correction, 5 June, 10:19 a.m.: The article has been corrected to state that six other sweeteners, both artificial and natural, had no impact on lifespan. An earlier version incorrectly stated that the other six sweeteners were all artificial.
Dogs, most of us think, have the best noses on the planet. But a new study reveals that this honor actually goes to elephants. The power of a mammal’s sniffer hinges on the number and type of its olfactory receptor genes. These genes are expressed in sensory cells that line the nasal cavity and are specialized for detecting odor molecules. When triggered, they set off a cascade of signals from the nose to the brain, enabling an animal to identify a particular smell. In the new study, scientists identified and examined olfactory receptor genes from 13 mammalian species. The researchers found that every species has a highly unique variety of such genes: Of the 10,000 functioning olfactory receptor genes the team studied, only three are shared among the 13 species. Perhaps not surprisingly, given the length of its trunk, the African elephant has the largest number of such genes—nearly 2000, the scientists report online today in the Genome Research. In contrast, dogs have only 1000, and humans and chimpanzees, less than 400—possibly because higher primates rely more on their vision and less on their sense of smell. The discovery fits with another recent study showing that Asian elephants are as good as mice (which have nearly 1300 olfactory receptor genes) at discriminating between odors; dogs and elephants have yet to be put to a nose-to-trunk sniffer test. Other research has also shown just how important a superior sense of smell is to the behemoths. A slight whiff is all that’s necessary, for instance, for elephants, such as those in the photo above, to distinguish between two Kenyan ethnic groups—the Maasai, who sometimes spear them, and the Kamba, who rarely pose a threat. They can also recognize as many as 30 different family members from cues in their urine.
The US today welcomed India’s ratification of the Paris climate agreement with President Barack Obama saying that by joining the pact Prime Minister Narendra Modi and the Indian people have carried on Mahatma Gandhi’s legacy. Related Items